Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea
Kong, H-Hee.; Shin, J-Yeol.; Yu, H-Sun.; Kim, J.; Hahn, T-Won.; Hahn, Y-Ho.; Chung, D-Il.
Journal of Clinical Microbiology 40(4): 1199-1206
ISSN/ISBN: 0095-1137 PMID: 11923331 DOI: 10.1128/jcm.40.4.1199-1206.2002
We applied ribosomal DNA PCR-restriction fragment length polymorphism (RFLP) and mitochondrial DNA (mtDNA) RFLP analyses to 43 Acanthamoeba environmental isolates (KA/LH1 to KA/LH43) from contact lens storage cases in southwestern Korea. These isolates were compared to American Type Culture Collection strains and clinical isolates (KA/E1 to KA/E12) from patients with keratiti. Seven riboprint patterns were seen.