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Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea

Kong, H-Hee.; Shin, J-Yeol.; Yu, H-Sun.; Kim, J.; Hahn, T-Won.; Hahn, Y-Ho.; Chung, D-Il.

Journal of Clinical Microbiology 40(4): 1199-1206

2002

ISSN/ISBN: 0095-1137
PMID: 11923331
DOI: 10.1128/jcm.40.4.1199-1206.2002
Accession: 003848502
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We applied ribosomal DNA PCR-restriction fragment length polymorphism (RFLP) and mitochondrial DNA (mtDNA) RFLP analyses to 43 Acanthamoeba environmental isolates (KA/LH1 to KA/LH43) from contact lens storage cases in southwestern Korea. These isolates were compared to American Type Culture Collection strains and clinical isolates (KA/E1 to KA/E12) from patients with keratiti. Seven riboprint patterns were seen.

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