EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c


Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c



Bioscience Biotechnology and Biochemistry 66(2): 277-284



ISSN/ISBN: 0916-8451

PMID: 11999399

DOI: 10.1271/bbb.66.277

We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,018 nucleotides and includes an open reading frame encoding a polypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase activity toward glycolchitin and antifungal activity against Trichoderma sp. as the authentic RSC-c did. RSC-c mutants were subsequently constructed and characterized with respect to their chitinase and antifungal activities. Mutation of Glu67 to Gln completely abolished the chitinase activity and diminished the antifungal activity. Considerable decreases in both activities were observed in the mutations of Trp72 and Ser120 to Ala, and Glu89 to Gln. The roles of these residues in the catalytic event of RSC-c are discussed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003851721

Download citation: RISBibTeXText

Related references

Molecular cloning, functional expression, and mutagenesis of cDNA encoding class I chitinase from rye (Secale cereale) seeds. Bioscience Biotechnology and Biochemistry 68(2): 324-332, 2004

Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization. Journal of Experimental Botany 50(339): 1627-1628, 1999

Molecular cloning and functional expression of chitinase-encoding cDNA from the cabbage moth, Mamestra brassicae. Molecules and Cells 33(5): 439-447, 2012

Molecular cloning, functional expression, and mutagenesis of cDNA encoding a cysteine proteinase inhibitor from sunflower seeds. Journal of Biochemistry 124(5): 911-916, 1998

Cloning of a plastidic rye (Secale cereale) b-glucosidase cDNA and its expression in Escherichia coli. Physiologia Plantarum 118(3): 7-45, 2003

Cloning of a plastidic rye (Secale cereale) beta -glucosidase cDNA and its expression in Escherichia coli. Physiologia Plantarum 118(3): 337-345, 2003

CDNA cloning of cytoplasmic ribosomal protein S7 of winter rye (Secale cereale) and its expression in low-temperature-treated leaves. Biochimica et Biophysica Acta 1492(1): 276-279, 2000

Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis. Amino Acids 32(1): 69-78, 2007

Positions of disulfide bonds in rye (Secale cereale) seed chitinase-a. Bioscience Biotechnology and Biochemistry 64(6): 1313-1316, 2000

Cloning and functional expression of a chitinase cDNA from the common cutworm, Spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene. Insect biochemistry and molecular biology 31(6-7): 521-532, 2001

Molecular cloning and functional expression of cDNA encoding a mammalian inorganic pyrophosphatase. Journal of Biological Chemistry 267(34): 24641-24647, 1992

Molecular cloning and functional expression of a cDNA encoding the human V1b vasopressin receptor. Journal of Biological Chemistry 269(43): 27088-27092, 1994

Molecular cloning of pea cDNA encoding cycloartenol synthase and its functional expression in yeast. Biological and Pharmaceutical Bulletin 20(7): 770-775, 1997

Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor. Proceedings of the National Academy of Sciences of the United States of America 90(21): 10056-10060, 1993

Molecular cloning and functional expression of cDNA encoding the pig plasma phospholipid transfer protein. Journal of Lipid Research 38(7): 1473-1481, 1997