Molecular markers linked to the leaf rust resistance gene Lr29 in F2 wheat population
Motawei, M.I.; Barakat, M.N.; Milad, S.I.; Moustafa, M.A.; El Daoudi, Y.H.
Alexandria Journal of Agricultural Research 46(2): 57-62
ISSN/ISBN: 0044-7250 Accession: 003852340
The objective of this study was to identify RAPD markers linked to the leaf rust (Puccinia recondita) resistance gene Lr29. Using bulked segregant analysis in conjunction with RAPD analysis, the 700 bp polymorphic fragment revealed by Primer 7 was found to be linked to the leaf rust resistance gene in an F2 segregating population. Analysis of genetic segregation of the polymorphic marker (Primer 7700) tested in the F2 population fitted the expected 3:1 Mendelian ratio.