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Nested PCR assays for detection of Monilinia fructicola in stone fruit orchards and Botryosphaeria dothidea from pistachios in California


Nested PCR assays for detection of Monilinia fructicola in stone fruit orchards and Botryosphaeria dothidea from pistachios in California



Journal of Phytopathology 151(6): 312-322



ISSN/ISBN: 0931-1785

DOI: 10.1046/j.1439-0434.2003.00725.x

Nested polymerase chain reaction (PCR) assays were developed based on microsatellite regions for detection of M. fructicola, the causal agent of brown rot of stone fruits, and B. dothidea, the causal agent of panicle and shoot blight of pistachio. The nested PCR primers specific to M. fructicola were developed based upon the sequence of a species-specific DNA fragment amplified by microsatellite primer M13.

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Accession: 003859026

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Related references

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