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Novel stepwise gradient reversed-phase liquid chromatography separations of humic substances, air particulate humic-like substances and lignins



Novel stepwise gradient reversed-phase liquid chromatography separations of humic substances, air particulate humic-like substances and lignins



Journal of Chromatography, A 1012(1): 67-79



RP-HPLC using stepwise gradients of dimethylformamide (DMF) in buffered aqueous mobile phase and a wide-pore (30 nm) octadecylsilica column has been applied to the analysis of soil, peat and air particulate humic and humic-like substances (HSs), as well as lignin, in order to demonstrate the usefulness of the approach for their characterization even at trace concentration level.

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Accession: 003864317

Download citation: RISBibTeXText

PMID: 14509343

DOI: 10.1016/s0021-9673(03)01177-4


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