Nucleotide sequence of cryIA gene cloned from Btk isolate of Bacillus thuringiensis and comparison with cryIA(c) gene from B. thuringiensis subsp. kenyae
Nucleotide sequence of cryIA gene cloned from Btk isolate of Bacillus thuringiensis and comparison with cryIA(c) gene from B. thuringiensis subsp. kenyae
Budiani, A.; Santoso, D.
Menara Perkebunan 68(1): 10-19
2000
This paper reports sequencing of cryIA gene fragment encoding toxin domain isolated from Btk isolates of B. thuringiensis using PCR technique and cloned with pGEM-T vector. To determine the full sequence of the 2-kb gene fragment, a series of mutants unidirectionally deleted at the 5'-end were constructed. Mutation was done using Erase-a Base-System kit. Three DNA mutants with appropriate degree of deletion and the un-mutated DNA were chosen for sequencing.