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Optimization of RAPD technique system for melon (Cucumis melo L.) germplasms

Optimization of RAPD technique system for melon (Cucumis melo L.) germplasms

Journal of Southwest Agricultural University 22(6): 512-515

The amplification efficiency of different DNA isolation procedures were compared in an L9 (34) orthogonal experiment. The optimum conditions of amplification for melon (Cucumis melo L.), i.e. dNTP density, primer density, Mg2+ density, Tap DNA density, annealing temperature and others, were rapidly and effectively identified.

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