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Optimization of a one-step PCR assay for the diagnosis of Flavescence doree-related phytoplasmas in field-grown grapevines and vector populations

Optimization of a one-step PCR assay for the diagnosis of Flavescence doree-related phytoplasmas in field-grown grapevines and vector populations

Vitis 40(4): 213-217

ISSN/ISBN: 0042-7500

Field-infected grapevines and natural populations of Scaphoideus titanus have been analysed to detect group V phytoplasmas associated with flavescence doree in northwestern Italy using nested PCR. A first amplification driven by universal ribosomal primers R16SF2/R2 was followed by a second round assisted by R16(V)F1/R1 primers and subsequent RFLP analysis.

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Accession: 003871023

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Optimisation of a one-step PCR assay for the diagnosis of Flavescence doreerelated phytoplasmas in field-grown grapevines and vector populations. Vitis 40(4): 213-217, 2001

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