EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Phylogenetic relationships among Flavescence doree strains and related phytoplasmas determined by heteroduplex mobility assay and sequence of ribosomal and nonribosomal DNA


Phylogenetic relationships among Flavescence doree strains and related phytoplasmas determined by heteroduplex mobility assay and sequence of ribosomal and nonribosomal DNA



Plant Pathology 52(5): 663-672



ISSN/ISBN: 0032-0862

DOI: 10.1046/j.1365-3059.2003.00917.x

Heteroduplex mobility assay (HMA) and DNA sequencing were performed on Flavescence doree (FD) phytoplasma strains and related phytoplasmas belonging to the elm yellows group. Part of the ribosomal RNA gene operon and a nonribosomal DNA region were utilized for phylogenetic analyses. Two FD strains, FD92 and FD-D, detected in France and Italy, respectively, were identical in both DNA fragments, confirming previous results. Other FD strains were all very similar and most closely resembled ALY, an Italian alder phytoplasma. Phytoplasmas associated with German Palatinate grapevine yellows were shown to form a distinct subcluster, also different from the elm yellows phytoplasma subcluster. Strain disparities revealed by HMA and sequence data were mostly in agreement, highlighting the utility of HMA in differentiation and classification of phytoplasmas belonging to the same ribosomal RNA group. Reprinted by permission of the publisher.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003882719

Download citation: RISBibTeXText

Related references

Generation and characterization of monoclonal antibodies to Flavescence doree phytoplasma: Serological relationships and differences in electroblot immunoassay profiles of Flavescence doree and Elm yellows phytoplasmas. European Journal of Plant Pathology 102(8): 757-764, 1996

Occurrence and diversity of phytoplasmas detected in clematis and their relationships with grapevine flavescence doree phytoplasmas. Bulletin of Insectology 60(2): 327-328, 2007

Influence of extraction medium on Bois noir and Flavescence doree detection, using polyclonal and monoclonal antibodies against stolbur and Flavescence doree phytoplasmas. Agronomie 16(6): 355-365, 1996

Influence of extraction medium on black wood and flavescence doree detection, using polyclonal and monoclonal antibodies against stolbur and flavescence doree phytoplasmas. Agronomie 16(6): 355-365, 1996

Optimization of a one-step PCR assay for the diagnosis of Flavescence doree-related phytoplasmas in field-grown grapevines and vector populations. Vitis 40(4): 213-217, 2001

Genetic variability among isolates of Coconut lethal yellowing phytoplasmas determined by Heteroduplex Mobility Assay. Tropical Plant Pathology: 5, 377-380, 2008

Diversity of phytoplasmas isolated from insects, determined by a DNA heteroduplex mobility assay and a length polymorphism of the 16S-23S rDNA spacer region analysis. Journal of applied microbiology 89(5): 744-750, 2000

Single and mixed infection by phytoplasmas of three 16S ribosomal subgroups (IB: aster yellows, VC: flavescence doree and XIIA: stolbur) in grapevines of southeastern Piemonte. Informatore Fitopatologico 51(9): 58-63, 2001

Identification and Epidemic Distribution of Two Flavescence Dorée-Related Phytoplasmas in Veneto (Italy). Plant Disease 83(10): 925-930, 1999

Grapevine yellows in Northern Italy: molecular identification of Flavescence dorée phytoplasma strains and of Bois Noir phytoplasmas. Journal of Applied Microbiology 103(6): 2325-2330, 2007

Revised Subgroup Classification of Group 16SrV Phytoplasmas and Placement of Flavescence Dorée-Associated Phytoplasmas in Two Distinct Subgroups. Plant Disease 85(7): 790-797, 2001

Heteroduplex mobility assay as a tool for predicting phylogenetic affiliation of environmental ribosomal RNA clones. Journal of Microbiological Methods 41(2): 155-160, 2000

Detection and diversity of flavescence doree - related phytoplasmas in alders surrounding infected vineyards in Aquitaine (France). Bulletin of Insectology 60(2): 329-330, 2007

Genetic relationships determined by a DNA heteroduplex mobility assay: analysis of HIV-1 env genes. Science 262(5137): 1257-1261, 1993

Evidence for the physical integrity of flavescence doree phytoplasmas purified by immunoaffinity from infected plants or leafhoppers and the plant pathogenicity of phytoplasmas from leafhoppers. Plant Pathology 44(6): 971-978, 1995