Propagation of Ilex aquifolium by stem cuttings for conservation and ornamental purposes

Spanos, K.; Gertsis, A.; Karalivanos, A.; Giakzidis, G.; Kottis, D.

Lesotekhnicheski Universitet Yubileen sbornik nauchni dokladi 75 godini visshe lesotekhnichesko obrazovanie v B"lgariya Sektsiya Ekologiya i opazvane na okolnata sreda: 40-45


Accession: 003900159

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Seven well developed, well formed, healthy genotypes of Ilex aquifolium were identified at Chortiatis mountain, close to the city of Thessaloniki in Greece, and selected for collection of cuttings. Stem cuttings of all 7 genotypes were successfully rooted up to 91% under a mist system in a shade house. Two types of stem cuttings were tested - semihardwood and hardwood. No significant difference was recorded between the two types of cuttings for overall rooting (average 69.5% and 67.3% for semihardwood and hardwood cuttings, respectively). However, highly significant differences were found between the different genotypes. For the semihardwood cuttings, genotypes 3 and 4 gave the highest rooting rate (91.0% and 87.61%, respectively) and genotype 7 gave the lowest rooting rate (54.4%). For the hardwood cuttings maximum rooting was with genotype 4 (89.5%) and minimum with genotype 7 (7.0%). In respect of bud flushing, significant differences were found between semihardwood and hardwood cuttings (average 23.3% and 39.1%, respectively) and also between different genotypes. For the hardwood cuttings maximum flushing rate was for genotype 3 (51.5%) and minimum for genotype 7 (4.0%) whereas for the semihardwood cuttings maximum flushing was recorded for genotype 4 (35.4%) and minimum for genotype 7 (13.3%). Propagation by stem cuttings therefore proved to be a successful regeneration method for conservation of this threatened species.