EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae


Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae



Current Microbiology 43(6): 448-451



ISSN/ISBN: 0343-8651

PMID: 11685515

DOI: 10.1007/s002840010337

The mosquito-larvicidal binary toxin of Bacillus sphaericus 2297 was expressed in Enterobacter amnigenus, a Gram-negative bacterium isolated from Anopheles dirus larvae gut. The toxin was placed under the regulation of various promoters in order to improve the expression level of the toxin. Amongst the recombinants obtained, E. amnigenus harboring pBS373, a plasmid which contains the toxin genes under the control of the native B. sphaericus promoter, expressed a significant amount of protein, comparable to that found in B. sphaericus 2297. In addition, this recombinant provided approximately twenty times higher toxicity against second-instar Anopheles dirus larvae when compared to B. sphaericus 2297. The procedure of obtaining this environmentally isolated bacterium from larvae gut and introducing the system for mosquito-larvicidal toxin synthesis is noteworthy. The promising result presented here provides a substantial degree of confidence for further field studies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003908611

Download citation: RISBibTeXText

Related references

The naturally derived insecticide spinosad is highly toxic to Aedes and Anopheles mosquito larvae. Medical and Veterinary Entomology 18(1): 50-56, 2004

Natural antibiotic susceptibility of Enterobacter amnigenus, Enterobacter cancerogenus, Enterobacter gergoviae and Enterobacter sakazakii strains. Clinical Microbiology and Infection: the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 8(9): 564-578, 2002

Stable integration and expression of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus into the chromosome of Enterobacter amnigenus: a potential breakthrough in mosquito biocontrol. FEMS Microbiology Letters 221(2): 243-248, 2003

Expression of mosquito-larvicidal toxin genes under the control of a native promoter in Enterobacter amnigenus An11. Biotechnology Letters 35(8): 1331-1337, 2013

The impact of transfluthrin on the spatial repellency of the primary malaria mosquito vectors in Vietnam: Anopheles dirus and Anopheles minimus. Malaria Journal 19(1): 9, 2020

The toxic effect of permethrin-impregnated bednets on Anopheles sinensis and Anopheles dirus. Acta Parasitologica et Medica Entomologica Sinica (First Issue): 49-54, 1993

Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi. Journal of Microbiological Methods 49(3): 329-334, 2002

Laboratory observation on Anopheles dirus instar 4 larva eating four species of Anopheles larvae. Sichuan Journal of Zoology 113: 31-32, 1992

Toxic effect of mangrove plant extracts on mosquito larvae Anopheles stephensi L. Current Science, India 57(16): 914-915, 1988

Characterization of the major form of glutathione transferase in the mosquito Anopheles dirus A. Biochemical Society Transactions 23(1): 81s, 1995

Purification and characterization of a major glutathione S-transferase from the mosquito Anopheles dirus (species B). Insect Biochemistry and Molecular Biology 26(3): 277-285, 1996

Deoxyribonucleic Acid Relatedness Between Enterobacter cloacae and Enterobacter amnigenus sp. nov. International Bulletin of Bacteriological Nomenclature and Taxonomy 31(1): 35-42, 1981

Isolation and Identification of Two l-Azetidine-2-carboxylic Acid-Degrading Soil Microorganisms, Enterobacter agglomerans and Enterobacter amnigenus. Journal of Natural Products 61(2): 207-211, 1998

Dna relatedness between enterobacter cloacae and enterobacter amnigenus new species. International Journal of Systematic Bacteriology 31(1): 35-42, 1981

New Bacillus thuringiensis strains isolated in Israel are highly toxic to mosquito larvae. Journal of Invertebrate Pathology 48(2): 216-222, 1986