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Resazurin reduction test to evaluate semen quality

Resazurin reduction test to evaluate semen quality

Veterinarske Novice 29(4): 183-186

The resazurin reduction test depends on the ability of the metabolically active spermatozoa to reduce the resazurin redox dye to resorufin, which is manifested as a visual colour change from blue (resazurin) to pink (resorufin) and further to white (dihydroresorufin). This test is a simple, reliable, and reproducible method for evaluating semen samples. The colour change of resazurin is matched with a colour chart, consisting of a spectrum of colours from blue to pink.

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