+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Specific RNA interference in psbP genes encoded by a multigene family in Nicotiana tabacum with a short 3'-untranslated sequence

Specific RNA interference in psbP genes encoded by a multigene family in Nicotiana tabacum with a short 3'-untranslated sequence

Bioscience Biotechnology and Biochemistry 67(1): 107-113

ISSN/ISBN: 0916-8451

PMID: 12619681

DOI: 10.1271/bbb.67.107

RNA interference with double-stranded RNA is a new method for the study of gene function in various organisms. In this report, we show that an inverted repeat of a short (103-bp) 3'-untranslated sequence of an isogene, 1A, of psbP genes, encoded by a small multigene family of four genes (1A, 2AF, 3F, and 5B) in Nicotiana tabacum, can specifically suppress the expression of psbP isogenes 1A and 5B with a 3'-untranslated sequence similar to a transcribed double-stranded RNA. The expression of other psbP isogenes, 2AF and 3F, was not affected, although the coding sequences of the psbP family genes are highly conserved. Consistent with this observation, small interfering RNAs were detected for the 3'-untranslated sequence used for the inverted-repeat transgene, and not for the coding sequence. These results suggest that double-stranded RNA having a 3'-untranslated sequence could be useful for an isogene-specific RNA interference of the family genes in Nicotiana tabacum.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003941564

Download citation: RISBibTeXText

Related references

Functional analysis of four members of the PsbP family in photosystem II in Nicotiana tabacum using differential RNA interference. Plant and Cell Physiology 46(12): 1885-1893, 2005

Nuclear-encoded chloroplast ribosomal protein L27 of Nicotiana tabacum: cDNA sequence and analysis of mRNA and genes. Biochemistry 31(29): 6856-6864, 1992

Short Exogenous Peptides Regulate Expression of CLE, KNOX1, and GRF Family Genes in Nicotiana tabacum. Biochemistry. Biokhimiia 82(4): 521-528, 2017

Cybridization in Nicotiana tabacum L. using double inactivation of parental protoplasts and post-fusion selection based on nuclear-encoded and chloroplast-encoded marker genes. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 88(8): 1017-1022, 1994

Study of the back-crossing effect of Nicotiana tabacum in hybrids obtained with the amphidiploids Nicotiana tabacum L. x Nicotiana debneyi D. (2n=96) and Nicotiana goodspeedii W. x Nicotiana tabacum L. (2n=88). Doklady Bolgarskoi akademii nauk = Comptes rendus de l'Academie bulgare des sciences 3(10): 1417-1420, 1980

Chromosome pairing and pollen fertility in the interspecific f 1 hybrids nicotiana tabacum l. x nicotiana benavidesii goodspeed nicotiana knightiana goodspeed x nicotiana tabacum and nicotiana raimondii macbride x nicotiana tabacum. Genetica Polonica 28(3): 263-270, 1987

Crystal structure of the PsbP protein of photosystem II from Nicotiana tabacum. Embo Reports 5(4): 362-367, 2004

Selection of plastid- and nuclear-encoded reference genes to study the effect of altered endogenous cytokinin content on photosynthesis genes in Nicotiana tabacum. Photosynthesis Research 102(1): 21-29, 2009

The heat-treatment induced reduction of the pat gene encoded herbicide resistance in Nicotiana tabacum is influenced by the transgene sequence. Journal of Plant Physiology 153(5-6): 631-642, 1998

Interspecific hybrids in nicotiana through in vitro culture of fertilized ovules nicotiana stocktonii x nicotiana tabacum sexual hybrid nicotiana nesophila x nicotiana tabacum sexual hybrid nicotiana repanda x nicotiana tabacum sexual hybrid. Journal of Heredity 69(5): 311-315, 1978

Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. Journal of Biological Chemistry 263(26): 13246-13251, 1988

Cyto genetics of a tri specific hybrid nicotiana benthamiana x nicotiana glutinosa x nicotiana tabacum and its allo poly ploids in genus nicotiana. Cytologia 42(3-4): 703-710, 1977

The tRNA(Tyr) multigene family of Nicotiana rustica: genome organization, sequence analyses and expression in vitro. Plant Molecular Biology 20(5): 869-878, 1992

Novel multigene family of variant genes encoded in the subtelomeric region of Plasmodium vivax. Memorias do Instituto Oswaldo Cruz 95(Suppl 2): 26, 2000

Characterization of genes specific to sua-CMS in Nicotiana tabacum. Plant Cell Reports 37(9): 1245-1255, 2018