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Studies on detection of swine transmissible gastroenteritis virus by reverse transcription-polymerase chain reaction (RT-PCR)


Studies on detection of swine transmissible gastroenteritis virus by reverse transcription-polymerase chain reaction (RT-PCR)



Chinese Journal of Veterinary Medicine 39(3): 19-20



It was found that the optimum Mg2+ concentration was 1.5 mol/litre for the fractional-step method and 2.0 mol/litre for the one-step method when using RT-PCR to detect swine transmissible gastroenteritis virus (TGEV). The fractional method could detect a 10-5/0.2 ml sample (12 PFU virus) and the one-step method could detect 10-4/0.2 ml sample (120 PFU virus).

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