Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis
Garaizar, J.; López-Molina, N.; Laconcha, I.; Lau Baggesen, D.; Rementeria, A.; Vivanco, A.; Audicana, A.; Perales, I.
Applied and Environmental Microbiology 66(12): 5273-5281
ISSN/ISBN: 0099-2240 PMID: 11097902 DOI: 10.1128/aem.66.12.5273-5281.2000
Strains of Salmonella enterica (n=212) of different serovars and phage types were used to establish a library typing computerized system for serovar Enteritidis on the basis of PCR fingerprinting, infrequent-restriction-site PCR (IRS-PCR), or pulsed-field gel electrophoresis (PFGE). The rate of PCR fingerprinting interassay and intercenter reproducibility was low and was only increased when DNA samples were extracted at the same time and amplified with the same reaction mixtures. Reproducibility of IRS-PCR technique reached 100%, but discrimination was low (D=0.52). The PFGE procedure showed an intercenter reproducibility value of 93.3%.