Survival against exogenous hydrogen peroxide of Photobacterium damselae subsp. piscicida under different culture conditions

Díaz-Rosales, P.; Chabrillón, M.; Moriñigo, M.A.; Balebona, M.C.

Journal of Fish Diseases 26(5): 305-308

2003


ISSN/ISBN: 0140-7775
PMID: 12962239
DOI: 10.1046/j.1365-2761.2003.00455.x
Accession: 003954898

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Abstract
The resistance to peroxide radicals of P. damselae subsp. piscicida cells grown under iron limited and repleted conditions, and pulsed with hydrogen peroxide was evaluated in this study. P. damselae subsp. piscicidia showed increase survival when exposed to peroxide radicals when cells have previously in contact with hydrogen peroxide. Both the virulent and non-virulent strains were inactivated after 1 h incubation with 10 mM hydrogen peroxide, however, when decreasing concentrations of peroxide were used, a higher degree of resistance to peroxide was observed in the virulent strain compared with the non-virulent strain. When bacteria were cultured under iron limited conditions, a significant decrease in survival was observed for both strains compared with cell grown under iron replete conditions or pulsed with peroxide. The decrease in bacterial survival in cultures grown under iron limited conditions suggests the presence of an iron-cofactored catalase in P. damselae subsp. piscicidia. Additional studies to demonstrate the presence of iron as a cofactor in the catalase, and the sensitivity of P. damselae subsp. piscicidia to the radicals generated during the macrophage respiratory burst, are in progress.