+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers



Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers



Eukaryotic Cell 2(1): 84-94



African trypanosomes cause sleeping sickness in humans and Nagana in cattle. The parasites multiply in the blood and escape the immune response of the infected host by antigenic variation. Antigenic variation is characterized by a periodic change of the parasite protein surface, which consists of a variant glycoprotein known as variant surface glycoprotein (VSG). Using a SELEX (systematic evolution of ligands by exponential enrichment) approach, we report the selection of small, serum-stable RNAs, so-called aptamers, that bind to VSGs with subnanomolar affinity. The RNAs are able to recognize different VSG variants and bind to the surface of live trypanosomes. Aptamers tethered to an antigenic side group are capable of directing antibodies to the surface of the parasite in vitro. In this manner, the RNAs might provide a new strategy for a therapeutic intervention to fight sleeping sickness.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003957928

Download citation: RISBibTeXText

PMID: 12582125

DOI: 10.1128/ec.2.1.84-94.2003


Related references

Serum-stable RNA aptamers to an invariant surface domain of live African trypanosomes. Combinatorial Chemistry & High Throughput Screening 9(7): 491-499, 2006

Biological variation among african trypanosomes: I. Clonal expression of virulence is not linked to the variant surface glycoprotein or the variant surface glycoprotein gene telomeric expression site. Dna and Cell Biology 29(5): 215-227, 2010

Subcellular Localization of a Variable Surface Glycoprotein Phosphatidylinositol-Specific Phospholipase-C in African Trypanosomes. The Journal of Cell Biology 105(2): 737-746, 1987

Subcellular localization of a variable surface glycoprotein phosphatidylinositol-specific phospholipase-C in African trypanosomes. Journal of Cell Biology 105(2): 737-746, 1987

Surface labeling of variable surface glycoprotein on viable african trypanosomes. Journal of Cellular Biochemistry Suppl. (10 Part A): 169, 1986

Microbial surface elements: the case of variant surface glycoprotein (VSG) genes of African trypanosomes. Genetic engineering principles and methods(3): 129-155, 1981

Differences in resistance to African trypanosomes are not directly linked to variant surface glycoprotein specific TH cell responses. FASEB Journal 10(6): A1183, 1996

Genetics of resistance to the African trypanosomes. VI. Heredity of resistance and variable surface glycoprotein-specific immune responses. Journal of Immunology 140(1): 283-288, 1988

The variant surface glycoprotein as a tool for adaptation in African trypanosomes. Microbes and Infection 8(3): 930-937, 2006

Control of variant surface glycoprotein messenger rna synthesis in african trypanosomes. Journal of Cellular Biochemistry Suppl. (13 Part E): 65, 1989

Maintaining the protective variant surface glycoprotein coat of African trypanosomes. Biochemical Society Transactions 33(Pt 5): 981-982, 2005

Identification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes. Experimental Parasitology 115(2): 173-180, 2007

Characterization of t helper cell responses to the variant surface glycoprotein vsg of african trypanosomes. FASEB Journal 6(4): A1220, 1992

On the role of repeated sequences 5' to variant surface glycoprotein genes in African trypanosomes. Gene 53(1): 55-62, 1987

The generation of order and genetic diversity in variable surface glycoprotein expression by African trypanosomes. Unknown, 1988