The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment

Duong Tan Nhut; Nguyen Thi Dieu Huong; Bui Van Le; Silva, J.T. da; Fukai, S.; Tanaka, M.

Journal of Horticultural Science and Biotechnology 77(1): 79-82

2002


ISSN/ISBN: 1462-0316
DOI: 10.1080/14620316.2002.11511461
Accession: 003964429

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Abstract
An efficient system has been developed for the in vitro shoot regeneration of L. longiflorum cv. Nellie White by culturing pretreated protocorm-like bodies (PLBs) derived from young stem transverse thin cell layers (tTCLs) exposed to various treatments of different medium volumes, pH, light intensity and sucrose concentrations. After 30 days of culture in 50 ml plastic petri dishes containing Murashige and Skoog medium supplemented with 0.2 mg thidiazuron (TDZ)/litre, the explants formed PLBs in all treatments. PLBs with the highest fresh weight were obtained after 30 days of culture when explants were cultured on MS medium containing 0.2 g TDZ/litre with either 25 ml volume, pH 6.1, 20 micro mol m-2 s-1 light intensity, or with 30 g sucrose/litre supplement. However, most shoot formation from PLBs was found using MS hormone-free medium with either 30 ml volume, pH 5.0, 40 micro mol m-2 s-1 light intensity, or with 20 g sucrose/litre after 30 days of culture. The highest shoot regeneration of L. longiflorum was derived from PLBs when explants were exposed to various media, indicating that the choice of explants and pretreatment in the medium with optimum volume, pH, light intensity and sucrose concentration are needed.