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The establishment of serum-free pig oocytes in vitro culture model


The establishment of serum-free pig oocytes in vitro culture model



Acta Agriculturae Shanghai 17(4): 99-101



In this study pig oocyte natural maturation and the effect of hypoxanthine (HX) on pig oocyte maturation were investigated, and a serum-free pig oocyte culture model in vitro was established mimic follicular environment in vivo. The results demonstrated that pig cumulus cell-enclosed oocyte (CEO) and denuded oocyte (DO) could resume meiosis spontaneously cultured in the serum-free M-199 environment.

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