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The use of a simplified DAS-ELISA procedure for large-scale detection of Apple chlorotic leaf spot and Apple stem grooving viruses in apple trees over extended sampling periods



The use of a simplified DAS-ELISA procedure for large-scale detection of Apple chlorotic leaf spot and Apple stem grooving viruses in apple trees over extended sampling periods



Acta Horticulturae (550 (Vol. 1)): 269-274



ACLSV is the most frequently encountered virus of apple orchards. As antisera of high quality are commercially available this should be a virus suited for routine detection by ELISA. However, different studies using DAS-ELISA have shown that, to be reliable, ACLSV detection should be done during a limited period in the growing season. In an attempt to overcome this limitation, we have conducted a series of experiments using different apple (cv.

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