Transformation of Solanum tuberosum L. with potato leafroll virus (PLRV) coat protein gene using Agrobacterium tumefaciens and phenotypic variation of transformed plants
Park YoungDoo; James, H.L.
Journal of the Korean Society for Horticultural Science 43(5): 501-506
2002
Accession: 003991864
Leaf segments of the United States genotypes, including established cultivars (Norchip, Russet Norkotah, Goldrush, and Norqueen Russet) and a breeding line (ND860-2) of potato (Solanum tuberosum) were inoculated with Agrobacterium tumefaciens that carried a binary vector, containing the coat protein (CP) gene of potato leafroll virus (PLRV). After inoculation, calluses and shoots were formed from leaf segments in the presence of kanamycin.