+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches

Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches

Veterinary Microbiology 94(1): 57-69

Escherichia coli is commonly isolated in canine pyometra, but little is known of the virulence factors that may be involved in the precipitation of this disease. The aim of this study was to compare the prevalence of uropathogenic virulence factor (UVF) genes in E. coli isolates from canine pyometra and from feces of healthy bitches to evaluate their role in the pathogenesis of pyometra. E. coli from 23 cases of canine pyometra and from the feces of 24 healthy bitches were analyzed, by polymerase chain reaction, for UVF genes associated with canine and human urinary tract infections (UTIs). The prevalences of UVFs in E. coli from canine pyometra were similar to that in canine and human uropathogenic E. coli. The prevalence of pap was greater (P=0.036) for E. coli from pyometra (52%) than for fecal isolates (21%), and the papGIII allele was present in all pap-containing isolates. The prevalences of genes for alpha-haemolysin and cytotoxic necrotising factor 1 were not significantly higher (P=0.075) in E. coli from pyometra than from feces. The proportion of pyometra strains with >or=3 UVFs was higher (P=0.039) than that of fecal strains, suggesting that possession of >or=3 UVF genes enhances the pathogenicity of the strain. Our findings demonstrate that E. coli associated with canine pyometra are similar to uropathogenic strains, and that operons that encode P fimbriae, alpha-haemolysin and cytotoxic necrotising factor 1 probably enhance the virulence and pathogenicity of the strain in the canine genital tract.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003998041

Download citation: RISBibTeXText

PMID: 12742716

DOI: 10.1016/s0378-1135(03)00063-4

Related references

Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs. Research in Veterinary Science 86(2): 206-210, 2008

Microbiological study of intrauterine secretion from bitches with pyometra and research of virulence factors of Escherichia coli isolates. Arquivos do Instituto Biologico Sao Paulo 71(Suplemento, RAIB): 513-515, 2004

Biochemical phenotypes of Escherichia coli in dogs: comparison of isolates isolated from bitches suffering from pyometra and urinary tract infection with isolates from faeces of healthy dogs. Veterinary Microbiology 52(3/4): 293-300, 1996

Virulence genotypes of Escherichia coli canine isolates from pyometra, cystitis and fecal origin. Veterinary Microbiology 166(3-4): 590-594, 2014

Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra. Veterinary Journal 202(2): 393-395, 2015

Decreased prevalence of virulence factors among ciprofloxacin-resistant uropathogenic Escherichia coli isolates. Journal of Clinical Microbiology 43(8): 4218-4220, 2005

Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains. International Journal of Microbiology 2014: 979584, 2014

Distribution of pathogenicity island markers and virulence factors in new phylogenetic groups of uropathogenic Escherichia coli isolates. Folia Microbiologica 63(3): 335-343, 2017

Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern. Osong Public Health and Research Perspectives 6(4): 261-268, 2015

Phylo-typing of clinical Escherichia coli isolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR. Veterinary Quarterly 35(4): 194-199, 2016

Enteroaggregative Escherichia coli with uropathogenic characteristics are present in feces of diarrheic and healthy children. Pathogens and Disease 75(8), 2017

Production of virulence factors by staphylococcus intermedius isolates from cases of canine pyoderma and healthy carriers. Microbial Ecology in Health & Disease 4(3): 169-174, 1991

Biofilm and fluoroquinolone resistance of canine Escherichia coli uropathogenic isolates. Bmc Research Notes 7: 499, 2015

Subtyping Escherichia coli Virulence Genes Isolated from Feces of Beef Cattle and Clinical Cases in Alberta. Foodborne Pathogens and Disease 14(1): 35-42, 2016

Virulence traits and pathogenicity of uropathogenic Escherichia coli isolates with common and uncommon O serotypes. Microbial Pathogenesis 104: 217-224, 2017