+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of salmonella isolates obtained from the carcasses and feces of swine at slaughter



Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of salmonella isolates obtained from the carcasses and feces of swine at slaughter



Applied and Environmental Microbiology 69(7): 4177-4182



Salmonella enterica isolates were recovered from swine at a collaborating processing plant over a 2-month period in the spring of 2000. In the present study, molecular subtyping by pulsed-field gel electrophoresis (PFGE) was performed on the 581 confirmed Salmonella isolates from the 84 Salmonella-positive samples obtained from the previous study. A total of 32 different PFGE pulsotypes were observed visually, and a BioNumerics software analysis clustered those pulsotypes into 12 PFGE groups. The B, F, and G groups predominated throughout the sampling period and were isolated from 39, 22, and 13% of the swine, respectively. In addition, multiple isolates were obtained from 67 of the 84 Salmonella-positive samples, and subtyping revealed multiple PFGE profiles in 35 of these 67 (62%) samples. Both carcass and fecal isolates of Salmonella were recovered from 13 swine, resulting in "matched" samples. Molecular typing of the 252 isolates recovered from the matched samples revealed that 7 (54%) of the 13 carcasses were contaminated with Salmonella pulsotypes that were not isolated from the feces of the same animal. Conversely, from 6 of the 13 (46%) matched animals, Salmonella clonal types were isolated from the feces that were not isolated from the carcass of the same animal. These data establish that each lot of swine introduces new contaminants into the plant environment and that swine feces from one animal can contaminate many carcasses. In addition, these results indicate that the examination of multiple Salmonella isolates from positive samples is necessary to determine the variety of potential contaminants of swine carcasses during slaughter and processing.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003999525

Download citation: RISBibTeXText

PMID: 12839796

DOI: 10.1128/AEM.69.7.4177-4182.2003


Related references

Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of Salmonella serotype Enteritidis, Typhimurium and Infantis isolates obtained from whole liquid eggs. International Journal of Food Microbiology 129(2): 180-186, 2009

Salmonella populations and prevalence in layer feces from commercial high-rise houses and characterization of the Salmonella isolates by serotyping, antibiotic resistance analysis, and pulsed field gel electrophoresis. Poultry Science 86(3): 591-597, 2007

Characterization of Salmonella isolates by serotype, antibiotic susceptibility test and pulsed field gel electrophoresis front the feces of laying hens in a commercial layer complex. Poultry Science 84: 14-14, 2005

Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan. Journal of Applied Microbiology 99(6): 1472-1483, 2005

Diversity of Campylobacter isolates from retail poultry carcasses and from humans as demonstrated by pulsed-field gel electrophoresis. Journal of Food Protection 65(6): 957-962, 2002

Differentiation of Campylobacter spp isolates from different broiler flocks and slaughter processing steps by pulsed-field gel electrophoresis. IJMM International Journal of Medical Microbiology 291(Supplement 31): 88, September, 2001

Combination of multilocus sequence typing and pulsed-field gel electrophoresis reveals an association of molecular clonality with the emergence of extensive-drug resistance (XDR) in Salmonella. Microbiological Research 207: 170-176, 2018

Combination of multilocus sequence typing and pulsed-field gel electrophoresis reveals an association of molecular clonality with the emergence of extensive-drug resistance (XDR) in Salmonella. Microbiological Research 207: 170-176, 2018

Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp. enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm. Veterinary Microbiology 99(2): 139-143, 2004

Use of pulsed-field gel electrophoresis to characterize the genetic diversity and clonal persistence of Salmonella senftenberg in mussel processing facilities. International Journal of Food Microbiology 105(2): 153-163, 2005

Chromosomal heterogeneity of Helicobacter pylori isolates by pulsed-field gel electrophoresis. Zentralblatt für Bakteriologie 280(1-2): 120-127, 1993

Heterogeneity of pneumocystis isolates by pulsed field gradient electrophoresis pfge. Abstracts of the General Meeting of the American Society for Microbiology 92: 510, 1992

Evaluation of pulsed-field gel electrophoresis and MIDI Sherlock the characterize a cluster of Stenotrophomonas maltophila isolates recovered from a medical center. Journal of Investigative Medicine 44(1): 191A, 1996

Salmonella on feces, hides and carcasses in beef slaughter facilities in Venezuela. International Journal of Food Microbiology 166(2): 226-230, 2013

Typing of Salmonella isolates from minced meat via pulsed-field gel electrophoresis. Archiv fur Lebensmittelhygiene 55(6): 134-136, 2004