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A reverse transcriptase-polymerase chain reaction assay for the diagnosis of turkey coronavirus infection



A reverse transcriptase-polymerase chain reaction assay for the diagnosis of turkey coronavirus infection



Journal of Veterinary Diagnostic Investigation 15(6): 592-596



This study reports on the development of a reverse transcriptase-polymerase chain reaction (RT-PCR) for the specific detection of turkey coronavirus (TCoV). Of the several sets of primers tested, 1 set of primers derived from the P gene and 2 sets derived from the N gene of TCoV could amplify the TCoV genome in the infected samples. The RT-PCR was sensitive and specific for TCoV and did not amplify other avian RNA and DNA viruses tested except the infectious bronchitis virus (IBV).

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Accession: 004022696

Download citation: RISBibTeXText

PMID: 14667027

DOI: 10.1177/104063870301500616


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