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A transgenic analysis of mouse lactate dehydrogenase c promoter activity in the testis






Journal of Andrology 24(6): 843-852

A transgenic analysis of mouse lactate dehydrogenase c promoter activity in the testis

Transcription of the mouse testis-specific lactate dehydrogenase c (mldhc) gene is limited to cells of the germinal epithelium. Cloning and analysis of the mldhc promoter revealed that a 100-bp core promoter was able to regulate testis-specific transcription in vitro and in transgenic mice. Surprisingly, expression of the reporter in transgenic testes was limited to pachytene spermatocytes, whereas native LDH-C(4) was detected in pachytene and all later germ cells. To locate additional regulatory sequence that could recapitulate the native LDH-C(4) distribution pattern, we investigated the contribution of 5' and 3' flanking sequences to the regulation of LDH-C(4) expression. We found that transcription factor YY1 binds to the mldhc promoter, that the mldhc 3' untranslated sequence does not permit a postmeiotic expression of a beta-galactosidase reporter in transgenic mice, and that native mldhc mRNA is predominately meiotic, with only a low level of postmeiotic distribution. Our results suggest that the high level of LDH-C(4) in postmeiotic cells results from mRNA and protein stability.

Accession: 004025584

PMID: 14581510

DOI: 10.1002/j.1939-4640.2003.tb03135.x

Download PDF Full Text: A transgenic analysis of mouse lactate dehydrogenase c promoter activity in the testis



Related references

Kroft, T.L.; Li, S.; Doglio, L.; Goldberg, E., 2003: A transgenic analysis of mouse lactate dehydrogenase C promoter activity in the testis. Transcription of the mouse testis-specific lactate dehydrogenase c (mldhc) gene is limited to cells of the germinal epithelium. Cloning and analysis of the mldhc promoter revealed that a 100-bp core promoter was able to regulate testis-specific tr...

Markert, C.L.; Amet, T.M.; Goldberg, E., 1998: Human testis-specific lactate dehydrogenase-C promoter drives overexpression of mouse lactate dehydrogenase-1 cDNA in testes of transgenic mice. The three isozymes of lactate dehydrogenase, each encoded by a separate gene, are developmentally regulated and differentially expressed in tissue-specific patterns. The lactate dehydrogenase-C (LDHC, mouse Ldh3) gene is temporally expressed exclu...

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