Section 5
Chapter 4,033

Agrobacterium rhizogenes is a useful transporter for introducing T-DNA of the binary plasmid into the chrysanthemum, Dendranthema grandiflorum Kitamura, genome

Tsuro, M.K.bo, T.S.izukawa, Y.T.kemoto, T.I.aba, K.

Plant cell tissue and organ culture 81(2): 175-181


ISSN/ISBN: 0167-6857
DOI: 10.1007/s11240-004-4783-1
Accession: 004032040

Agrobacterium rhizogenes was used for efficient transformation of chrysanthemum. Two types of Agrobacterium, A. rhizogenes (A-13) and A. tumefaciens (LBA4404), which harbor pIG121-Hm, were employed for infection. In the A. rhizogenes-infected explants, hairy roots were not observed on any tested medium or culture condition. When explants were cultured on shoot induction medium, calli were formed at the cutting edge within 4-6 weeks of culture, and shoot primordia were observed on the callus surface after 2 weeks of callus formation. Consequently, with gus introduction, a significantly higher transformation rate was observed for A. rhizogenes (6.0%) compared with A. tumefaciens (3.3%). However, only 0.6% of the frequency of rol insertion was exhibited in A. rhizogenes mediation. These results indicate that A. rhizogenes effectively introduces T-DNA of the binary plasmid into the chrysanthemum genome by introducing Ri T-DNA at a low frequency. It also indicates that the system is a useful alternative for the transformation of chrysanthemum.

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