+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Association of the kdr and superkdr sodium channel mutations with resistance to pyrethroids in Louisiana populations of the horn fly, Haematobia irritans irritans (L.)



Association of the kdr and superkdr sodium channel mutations with resistance to pyrethroids in Louisiana populations of the horn fly, Haematobia irritans irritans (L.)



Veterinary Parasitology 129(1-2): 149-158



Pyrethroid resistance in three horn fly populations in Louisiana was monitored by weekly fly counts, filter paper bioassays, and diagnostic PCR assays for the presence of pyrethroid resistance-associated mutations in the sodium channel gene coding region. The PCR assay for the knockdown resistance (kdr) and superkdr sodium channel mutations was used to determine the frequency of the target site insensitivity mechanism in the populations of horn flies, which possessed varying degrees of insecticide resistance. The bioassays and frequency of homozygous-resistant (RR) kdr genotypes were relative predictors of the fly control subsequently observed. Flies exposed to filter paper impregnated with a discriminating concentration of one of four different insecticides were collected when 50% mortality was estimated. Genotypes for the dead flies and the survivors were determined by the PCR assay. The results of the PCR assays indicated that the genotype at the kdr locus of the flies exposed to the two pyrethroids had an effect upon whether the flies were considered to be alive or dead at the time of collection. The kdr genotype of flies exposed to either diazinon or doramectin was unrelated to whether the flies were considered to be alive or dead, except for a single comparison of flies exposed to diazinon. When possible interactions of the kdr and superkdr mutations were compared, we found that there were no associations with the response to diazinon and doramectin. For one location, there were no survivors of the 75 flies with the SS-SS (superkdr-kdr) homozygous susceptible wild type genotype exposed to pyrethroids, while there were survivors in all of the other five genotypes. The SS-RR genotype flies were more susceptible to the pyrethroids than the SR-RR flies, but that was not the case for exposure to diazinon or doramectin. In the St. Joseph population, there were an adequate number of flies to demonstrate that the SS-SR genotype was more susceptible to pyrethroids than the SS-RR and that flies with the SR-SR genotype were more susceptible to pyrethroids than the flies with the SR-RR genotype.

(PDF emailed within 0-6 h: $19.90)

Accession: 004050449

Download citation: RISBibTeXText

PMID: 15817215

DOI: 10.1016/j.vetpar.2005.01.007



Related references

Screening of Haematobia irritans irritans (Diptera: Muscidae) populations for pyrethroid resistance-associated sodium channel gene mutations by using a polymerase chain reaction assay. Journal of Medical Entomology 35(5): 710-715, 1998

Role of the kdr and super-kdr sodium channel mutations in pyrethroid resistance: correlation of allelic frequency to resistance level in wild and laboratory populations of horn flies (Haematobia irritans). Insect Biochemistry and Molecular Biology 28(12): 1031-1037, 1998

Annotated bibliography of the horn fly, Haematobia irritans irritans (L.) including references on the buffalo fly, Haematobia irritans exigua (de meijere), and other species belonging to the genus Haematobia. 1977

Physiological age composition and reproductive biology of horn fly populations haematobia irritans irritans diptera muscidae in iowa usa. Journal of Medical Entomology 20(6): 664-669, 1983

Dynamics and mechanisms of permethrin resistance in a field population of the horn fly, Haematobia irritans irritans. Insect Science 16(2): 175-184, 2009

Behavioral resistance to the pyrethroids in the horn fly haematobia irritans diptera muscidae. Environmental Entomology 14(6): 873-880, 1985

Behavioural resistance to the pyrethroids in the horn fly, Haematobia irritans (Diptera: Muscidae). Environmental Entomology, 146: 873-880, 1985

Determination of plasma parameters associated with variation in resistance to horn fly (Haematobia irritans irritans L.) and their effect on beef cattle production. Zootecnia Tropical 19(1): 7-16, 2001

Survey of resistance to permethrin and diazinon and the use of a multiplex polymerase chain reaction assay to detect resistance alleles in the horn fly, Haematobia irritans irritans (L.). Journal of Medical Entomology 40(6): 942-949, 2003

Biological control of horn fly (Haematobia irritans irritans) in Selviria, Mato Grosso do Sul State. 3. Survey of fimicolous insects associated with the horn fly. Revista Brasileira de Entomologia 39(2): 249-258, 1995

Antennal olfactory sensilla of the horn fly, Haematobia irritans irritans (L.) (Diptera: Muscidae). Journal of the Kansas Entomological Society, 533: 641-652, 1980

Antennal olfactory sensilla of the horn fly haematobia irritans irritans diptera muscidae. Journal of the Kansas Entomological Society 53(3): 641-652, 1980

Susceptibility of the horn fly, Haematobia irritans irritans (Diptera: Muscidae), to insecticides in Brazil. Revista Brasileira de Parasitologia Veterinaria 21(2): 125-132, 2013

Bovine teat atresia associated with horn fly (Haematobia irritans irritans (L.))-induced dermatitis. Veterinary Pathology 37(4): 360-364, 2000

Courtship, mating, and ovarian development of the horn fly Haematobia irritans irritans (L.). Dissertation Abstracts International B Sciences and Engineering, 4110: 3681-3682, 1981