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BamHI restriction fragment length polymorphism within the env gene of bovine leukaemia provirus



BamHI restriction fragment length polymorphism within the env gene of bovine leukaemia provirus



Bulletin of the Veterinary Institute in Puawy 48(1): 15-18



BamHI restriction fragment length polymorphism (RFLP) method was applied to analyse DNA provirus in cattle naturally infected with bovine leukaemia virus (BLV). The 341 bp DNA (fragment) of BLV env gene PCR-amplified and subjected to digestion with BamHI restriction enzyme gave different RFLP patterns (341 bp env fragment was not cut (-), digested into two fragments of 162 bp and 178 bp length (+), or partially digested (+/-)).

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Accession: 004053015

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