+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterisation of a Cryptosporidium isolate from water buffalo (Bubalus bubalis) by sequencing of a fragment of the Cryptosporidium oocyst wall protein gene (COWP)



Characterisation of a Cryptosporidium isolate from water buffalo (Bubalus bubalis) by sequencing of a fragment of the Cryptosporidium oocyst wall protein gene (COWP)



Veterinary Parasitology 131(1-2): 139-144



Cryptosporidium oocysts were detected using a direct immunofluorescence antibody test in the faeces of an asymptomatic water buffalo (Bubalus bubalis) heifer from a dairy farm close to Santiago de Compostela (NW Spain). Oocysts were morphologically indistinguishable from Cryptosporidium parvum. Using DNA extracted from this sample and a PCR-RFLP analysis of a 341 base pairs fragment of the Cryptosporidium oocyst wall protein (COWP) gene, a previously undescribed fragment pattern was generated. The COWP gene fragment was cloned and sequencing analyses revealed it to be similar to the C. parvum 'pig' genotype but with four base pairs substitutions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004068807

Download citation: RISBibTeXText

PMID: 15935559

DOI: 10.1016/j.vetpar.2005.04.022


Related references

The identification of Cryptosporidium species and Cryptosporidium parvum directly from whole faeces by analysis of a multiplex PCR of the 18S rRNA gene and by PCR/RFLP of the Cryptosporidium outer wall protein (COWP) gene. International Journal for Parasitology 29(8): 1241-1247 (Special Issue), 1999

Utility of the Cryptosporidium oocyst wall protein (COWP) gene in a nested PCR approach for detection infection in cattle. Veterinary Parasitology 111(2/3): 153-159, 2003

First molecular characterisation of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) in Victoria, Australia. Infection Genetics and Evolution 20: 96-102, 2013

Cloning of Cryptosporidium oocyst wall protein COWP-190. Journal of Eukaryotic Microbiology 41(1): 3A, 1994

Epitopes of major Cryptosporidium parvum protein COWP-190 expressed on inner face of the oocyst wall. Abstracts of the General Meeting of the American Society for Microbiology 97: 459, 1997

PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiology Letters 150(2): 209-217, 1997

Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo (Bubalus bubalis). Veterinary Parasitology 150(1-2): 146-149, 2007

Concurrent Cryptosporidium and Candida infections in a buffalo calf Bubalus bubalis. Comparative Clinical Pathology 22(5): 895-898, 2013

Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene. Emerging Infectious Diseases 7(1): 49-56, 2001

Direct sequencing of the water buffalo (Bubalus bubalis) kappa -casein gene. Animal Science and Technology 65(7): 652-655, 1994

Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis). Genetics and Molecular Research 11(3): 2878-2883, 2012

Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites. Applied and Environmental Microbiology 66(12): 5499-5502, 2000

Giardia and Cryptosporidium in water buffaloes (Bubalus bubalis). Parasitology Research 100(5): 1113-1118, 2007

First identification of Cryptosporidium parvum subtype IIaA20G1R1 in water buffalos ( Bubalus bubalis ). Research in Veterinary Science 118: 181-183, 2018

Cryptosporidium and Giardia in water buffaloes (Bubalus bubalis) of the Italian Mediterranean bred. Veterinary Research Communications 31(Suppl. 1): 253-255, 2007