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Cloning and expression of outer surface protein C gene from Borrelia burgdorferi PD91 strain and sequence analysis


Cloning and expression of outer surface protein C gene from Borrelia burgdorferi PD91 strain and sequence analysis



Zhongguo Meijieshengwuxue ji Kongzhi Zazhi = Chinese Journal of Vector Biology and Control 14(5): 329-331



An experiment was conducted to construct an expression vector carrying the outer surface protein C (ospC) gene of Borrelia burgdorferi. The organism was cloned and expressed to study the prevention, diagnosis and pathogenesis of Lyme disease. The ospC gene was amplified from the genome of B. burgdorferi PD91 strain by PCR and recombined with plasmid PET-11D. The recombinant plasmid PET-11D-ospC was identified with restriction endonuclease analysis and sequencing.

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