Cloning and fusion expression of human lactoferrin protein gene in prokaryotic expression vector

Li ShuQin; Zhang LiQun; Sun XueMei; Jia ShiQian; Zhou WeiMing; Zhang JianHua; Lu JiaPing; Zhang LieBing

China Dairy Industry 30(3): 3-5


Accession: 004076330

Download citation:  

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

By designing special primers, the human lactoferrin protein gene was amplified by PCR and cloned into the pGEX-4T3 vector. It was then transferred into Escherichia coli BL21; induced by IPTG, the fusion protein was expressed. Western Blot analysis indicated that the fusion protein was GST-human lactoferrin protein.