Cloning and fusion expression of human lactoferrin protein gene in prokaryotic expression vector

Li ShuQin; Zhang LiQun; Sun XueMei; Jia ShiQian; Zhou WeiMing; Zhang JianHua; Lu JiaPing; Zhang LieBing

China Dairy Industry 30(3): 3-5

2002


Accession: 004076330

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Abstract
By designing special primers, the human lactoferrin protein gene was amplified by PCR and cloned into the pGEX-4T3 vector. It was then transferred into Escherichia coli BL21; induced by IPTG, the fusion protein was expressed. Western Blot analysis indicated that the fusion protein was GST-human lactoferrin protein.