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Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells



Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells



Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 19(3): 260-263



Total RNA was extracted from the right atrial tissue of a patient with heart failure, and the full-length angiotensin converting enzyme 2 (ACE-2) gene was amplified by a nested reverse transcription-polymerase chain reaction method. The ACE-2 gene was cloned into the pcDNA4/HisMax-TOPO eukaryotic expression vector to construct the recombinant plasmid pcDNA4/ACE-2, and then transfected into 293 T lymphocytes. ACE-2 transient expression was examined by western blotting.

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Accession: 004076561

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PMID: 16261211


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