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Cloning of the promoter fragment of a cold-inducible gene (cor15a) from Arabidopsis thaliana and its expression in transgenic potato



Cloning of the promoter fragment of a cold-inducible gene (cor15a) from Arabidopsis thaliana and its expression in transgenic potato



Journal of Agricultural Biotechnology 12(5): 500-504



The promoter of a cold-inducible gene cor15a was amplified with PCR from the genome DNA of A. thaliana Columbia, which had a length of 900 bp and the same sequence as published previously. The expression vector pLB was constructed by inserting the cor15a promoter into the upstream of beta-glucuronidase (gus) gene followed by NOS terminator in pBI121.

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