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Comparison of alpha -galactosidase activity in cells and cultivation medium of lemon balm (Melissa officinalis L.)



Comparison of alpha -galactosidase activity in cells and cultivation medium of lemon balm (Melissa officinalis L.)



Acta Horticulturae et Regiotecturae 7(1): 17-19



A rapid, simple and reproducible method for the identification of extracellular alpha -galactosidase has been developed. The alpha -galactosidase secreted by callus culture or by roots of the seedlings hydrolyses the substrate (1-naphthyl- alpha -D-galactopyranoside) in alpha -D-galactose and 1-naphthol, the latter forming slightly soluble reddish brown or reddish violet azo-dye after simultaneous azocoupling with hexazonium p-rosaniline or Fast Blue B.

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Accession: 004081430

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