Comparison of fluorescence microscopy and solid-phase cytometry methods for counting bacteria in water
Lisle, J.T.; Hamilton, M.A.; Willse, A.R.; McFeters, G.A.
Applied and Environmental Microbiology 70(9): 5343-5348
Total direct counts of bacterial abundance are central in assessing the biomass and bacteriological quality of water in ecological and industrial applications. Several factors have been identified that contribute to the variability in bacterial abundance counts when using fluorescent microscopy, the most significant of which is retaining an adequate number of cells per filter to ensure an acceptable level of statistical confidence in the resulting data.