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Comparison of fluorescence microscopy and solid-phase cytometry methods for counting bacteria in water

Lisle, J.T.; Hamilton, M.A.; Willse, A.R.; McFeters, G.A.

Applied and Environmental Microbiology 70(9): 5343-5348

2004


ISSN/ISBN: 0099-2240
PMID: 15345419
DOI: 10.1128/aem.70.9.5343-5348.2004
Accession: 004082213

Total direct counts of bacterial abundance are central in assessing the biomass and bacteriological quality of water in ecological and industrial applications. Several factors have been identified that contribute to the variability in bacterial abundance counts when using fluorescent microscopy, the most significant of which is retaining an adequate number of cells per filter to ensure an acceptable level of statistical confidence in the resulting data.

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