+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A

Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A

Journal of General Virology 86(Pt 1): 91-105

A second genotype of Mamestra configurata nucleopolyhedrovirus-A (MacoNPV-A), variant 90/4 (v90/4), was identified due to its altered restriction endonuclease profile and reduced virulence for the host insect, M. configurata, relative to the archetypal genotype, MacoNPV-A variant 90/2 (v90/2). To investigate the genetic differences between these two variants, the genome of v90/4 was sequenced completely. The MacoNPV-A v90/4 genome is 153 656 bp in size, 1404 bp smaller than the v90/2 genome. Sequence alignment showed that there was 99.5 % nucleotide sequence identity between the genomes of v90/4 and v90/2. However, the v90/4 genome has 521 point mutations and numerous deletions and insertions when compared to the genome of v90/2. Gene content and organization in the genome of v90/4 is identical to that in v90/2, except for an additional bro gene that is found in the v90/2 genome. The region between hr1 and orf31 shows the greatest divergence between the two genomes. This region contains three bro genes, which are among the most variable baculovirus genes. These results, together with other published data, suggest that bro genes may influence baculovirus genome diversity and may be involved in recombination between baculovirus genomes. Many ambiguous residues found in the v90/4 sequence also reveal the presence of 214 sequence polymorphisms. Sequence analysis of cloned HindIII fragments of the original MacoNPV field isolate that the 90/4 variant was derived from indicates that v90/4 is an authentic variant and may represent approximately 25 % of the genotypes in the field isolate. These results provide evidence of extensive sequence variation among the individual genomes comprising a natural baculovirus outbreak in a continuous host population.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004084276

Download citation: RISBibTeXText

PMID: 15604435

DOI: 10.1099/vir.0.80488-0

Related references

Identification and genomic analysis of a second species of nucleopolyhedrovirus isolated from Mamestra configurata. Virology 297(2): 226-244, 2002

Complete genomic sequences and comparative analysis of Mamestra brassicae nucleopolyhedrovirus isolated in Korea. Virus Genes 47(1): 133-151, 2013

Biological and biochemical comparison of mamestra configurata and mamestra brassicae nuclear polyhedrosis virus isolates pathogenic for the bertha armyworm mamestra configurata lepidoptera noctuidae. Journal of Invertebrate Pathology 56(1): 47-56, 1990

Characterization of Mamestra configurata nucleopolyhedrovirus enhancin and its functional analysis via expression in an Autographa californica M nucleopolyhedrovirus recombinant. Journal of General Virology 84(Pt 1): 123-132, 2003

A physical map of the Mamestra configurata nucleopolyhedrovirus genome and sequence analysis of the polyhedrin gene. Journal of General Virology 78: 265-271, 1997

Sequence and organization of the Mamestra configurata nucleopolyhedrovirus genome. Virology 294(1): 106-121, 2002

Mamestra configurata nucleopolyhedrovirus-A transcriptome from infected host midgut. Virus Genes 48(1): 174-183, 2014

Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins. Journal of General Virology 93(Pt 4): 744-753, 2012

Comparative transcriptional and genomic analysis of Plasmodium falciparum field isolates. Plos Pathogens 5(10): E1000644, 2009

Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae. Journal of Applied Entomology e; 125(5): 267-270, 2001

Complete genomic sequence and comparative analysis of four genital and respiratory isolates of bovine herpesvirus subtype 1.2b (BoHV-1.2b), including the prototype virus strain K22. Archives of Virology 161(11): 3269-3274, 2016

Comparative genomic analysis of isolates belonging to the six species of the genus Thermus using pulsed-field gel electrophoresis and ribotyping. Archives Of Microbiology. 168(2): 92-101, 1997

Complete genomic, comparative genomic and post-genomic analysis of lactic acid bacteria. Journal of Azabu University 9/10: 250-254, 2004