+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Complex regulation of Arabidopsis AGR1/PIN2-mediated root gravitropic response and basipetal auxin transport by cantharidin-sensitive protein phosphatases



Complex regulation of Arabidopsis AGR1/PIN2-mediated root gravitropic response and basipetal auxin transport by cantharidin-sensitive protein phosphatases



Plant Journal 42(2): 188-200



Polar auxin transport, mediated by two distinct plasma membrane-localized auxin influx and efflux carrier proteins/complexes, plays an important role in many plant growth and developmental processes including tropic responses to gravity and light, development of lateral roots and patterning in embryogenesis. We have previously shown that the Arabidopsis AGRAVITROPIC 1/PIN2 gene encodes an auxin efflux component regulating root gravitropism and basipetal auxin transport. However, the regulatory mechanism underlying the function of AGR1/PIN2 is largely unknown. Recently, protein phosphorylation and dephosphorylation mediated by protein kinases and phosphatases, respectively, have been implicated in regulating polar auxin transport and root gravitropism. Here, we examined the effects of chemical inhibitors of protein phosphatases on root gravitropism and basipetal auxin transport, as well as the expression pattern of AGR1/PIN2 gene and the localization of AGR1/PIN2 protein. We also examined the effects of inhibitors of vesicle trafficking and protein kinases. Our data suggest that protein phosphatases, sensitive to cantharidin and okadaic acid, are likely involved in regulating AGR1/PIN2-mediated root basipetal auxin transport and gravitropism, as well as auxin response in the root central elongation zone (CEZ). BFA-sensitive vesicle trafficking may be required for the cycling of AGR1/PIN2 between plasma membrane and the BFA compartment, but not for the AGR1/PIN2-mediated root basipetal auxin transport and auxin response in CEZ cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004084428

Download citation: RISBibTeXText

PMID: 15807782

DOI: 10.1111/j.1365-313x.2005.02369.x


Related references

PINOID kinase regulates root gravitropism through modulation of PIN2-dependent basipetal auxin transport in Arabidopsis. Plant Physiology 150(2): 722-735, 2009

Pinoid Kinase Regulates Root Gravitropism through Modulation of Pin2-Dependent Basipetal Auxin Transport in Arabidopsis. Plant Physiology 150(2): 722-735, 2009

Ubiquitylation-mediated control of polar auxin transport: analysis of Arabidopsis PIN2 auxin transport protein. Methods in Molecular Biology 1209: 233-249, 2014

Aluminium toxicity targets PIN2 in Arabidopsis root apices Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport. Chinese Science Bulletin 53(16): 2480-2487, 2008

N-glycan containing a core α1,3-fucose residue is required for basipetal auxin transport and gravitropic response in rice (Oryza sativa). New Phytologist 212(1): 108-122, 2016

Inactivation of Plasma MembraneLocalized Cdpk-Related Kinase5 Decelerates Pin2 Exocytosis and Root Gravitropic Response in Arabidopsis. The Plant Cell 25(5): 1592-1608, 2013

Inactivation of plasma membrane-localized CDPK-RELATED KINASE5 decelerates PIN2 exocytosis and root gravitropic response in Arabidopsis. Plant Cell 25(5): 1592-1608, 2013

The Tomato 14-3-3 Protein Tft4 Modulates H+Efflux, Basipetal Auxin Transport, and the Pks5-J3 Pathway in the Root Growth Response to Alkaline Stress. Plant Physiology 163(4): 1817-1828, 2013

The Tomato 14-3-3 protein TFT4 modulates H+ efflux, basipetal auxin transport, and the PKS5-J3 pathway in the root growth response to alkaline stress. Plant Physiology 163(4): 1817-1828, 2013

Dorenone blocks polarized tip growth of root hairs by interfering with the PIN2-mediated auxin transport network in the root apex. Plant Journal: 4, 709-717, 2008

D'orenone blocks polarized tip growth of root hairs by interfering with the PIN2-mediated auxin transport network in the root apex. The Plant Journal 55(4): 709-717, 2008

The signal transducer NPH3 integrates the phototropin1 photosensor with PIN2-based polar auxin transport in Arabidopsis root phototropism. Plant Cell 24(2): 551-565, 2012

COP1 mediates the coordination of root and shoot growth by light through modulation of PIN1- and PIN2-dependent auxin transport in Arabidopsis. Development 139(18): 3402-3412, 2012

The Signal Transducer Nph3 Integrates the Phototropin1 Photosensor with Pin2-Based Polar Auxin Transport in Arabidopsis Root Phototropism. The Plant Cell 24(2): 551-565, 2012

Mutations in Arabidopsis multidrug resistance-like ABC transporters separate the roles of acropetal and basipetal auxin transport in lateral root development. Plant Cell 19(6): 1826-1837, 2007