+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Component-resolved in vitro diagnosis in carrot allergy: does the use of recombinant carrot allergens improve the reliability of the diagnostic procedure?



Component-resolved in vitro diagnosis in carrot allergy: does the use of recombinant carrot allergens improve the reliability of the diagnostic procedure?



Clinical and Experimental Allergy 35(7): 970-978



In Europe, pollen-related food allergy is the most frequent form of food allergy in adults. Reliability of current diagnostic procedures, however, is poor and therapeutic options are not available. In the present study, we created a panel of recombinant allergens from carrot and evaluated its potential in component-resolved in vitro diagnosis of carrot allergy. Recombinant (r) Dau c 1.0104, Dau c 1.0201 and Dau c 4 were cloned by a polymerase chain reaction strategy, expressed in Escherichia coli and purified. Carrot lipid transfer protein (LTP) was expressed in the yeast Pichia pastoris. Sera from 40 carrot-allergic patients were investigated. Twenty-one birch pollen-allergic subjects with negative open provocation to carrot and 20 non-allergic subjects were included as controls. IgE binding to recombinant allergens as well as to cross-reactive carbohydrate determinants (CCD) was measured by ELISA. Cross-reactivity between Dau c 1 isoforms and Bet v 1 was assayed by ELISA inhibition. Biological activity of the recombinant carrot allergens was assessed by histamine release assay and peripheral blood mononuclear cells stimulation. Ninety-eight percent of the carrot-allergic patients were positive to at least one recombinant allergen; 98% reacted to rDau c 1.0104, 65% to rDau c 1.0201, 38% to rDau c 4 and 20% had IgE against CCD. Specificity using the recombinant allergens was high when compared with non-allergic controls, but low compared with birch-sensitized subjects without carrot allergy. Sensitization to Dau c 1.0201, however, proved to be highly specific for clinically relevant sensitization. Inhibition assays indicated the absence of LTP in carrot root extract, and epitope diversity between Dau c 1.0104, Dau c 1.0201 and Bet v 1. Our panel of recombinant allergens from carrot can provide a standardized tool for in vitro diagnosis of carrot allergy, and for epitope studies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004084506

Download citation: RISBibTeXText

PMID: 16008686

DOI: 10.1111/j.1365-2222.2005.02294.x


Related references

Component-resolved in vitro diagnosis of carrot allergy in three different regions of Europe. Allergy 67(6): 758-766, 2012

Component resolved diagnosis in carrot allergy. 2007

Component-resolved diagnosis with recombinant allergens in patients with cherry allergy. Journal of Allergy and Clinical Immunology 110(1): 167-173, 2002

Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis. Molecular Biology Reports 39(3): 3169-3178, 2012

Component-resolved diagnosis (CRD) of type I allergy with recombinant grass and tree pollen allergens by skin testing. Journal of Investigative Dermatology 113(5): 830-837, 1999

Component-resolved diagnosis of kiwifruit allergy with purified natural and recombinant kiwifruit allergens. Journal of Allergy and Clinical Immunology 125(3): 687-94 694.E1, 2010

Component-resolved diagnosis of house-dust mite allergy with purified natural and recombinant mite allergens. Clinical and Experimental Allergy 34(4): 597-603, 2004

Diagnostic precision of component-resolved vs. extract-based in vitro diagnosis of hymenoptera venom allergy: effects on clinical management. Journal der Deutschen Dermatologischen Gesellschaft 15(5): 507-515, 2017

Defining specific allergens for improved component-resolved diagnosis of shrimp allergy in adults. Molecular Immunology 112: 330-337, 2019

Dau c 1.01 and Dau c 1.02-silenced transgenic carrot plants show reduced allergenicity to patients with carrot allergy. Transgenic Research 20(3): 547-556, 2011

First Report of Carrot red leaf virus and Carrot mottle virus, Causal Agents of Carrot Motley Dwarf, in Carrot in Mauritius. Plant Disease 93(11): 1218, 2009

Component-resolved diagnosis of pollen allergy based on skin testing with profilin, polcalcin and lipid transfer protein pan-allergens. Clinical and Experimental Allergy 39(11): 1764-1773, 2009

Characterization of recombinant Mal d 4 and its application for component-resolved diagnosis of apple allergy. Clinical and Experimental Allergy 36(8): 1087-1096, 2006

Floating rowcovers improve germination and reduce carrot weevil infestations in carrot. Hortscience: 5, 1619-1622, 2008

Carrot allergy: double-blinded, placebo-controlled food challenge and identification of allergens. Journal of Allergy and Clinical Immunology 108(2): 301-307, 2001