+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Composition and structure of the centromeric region of rice chromosome 8



Composition and structure of the centromeric region of rice chromosome 8



Plant Cell 16(4): 967-976



Understanding the organization of eukaryotic centromeres has both fundamental and applied importance because of their roles in chromosome segregation, karyotypic stability, and artificial chromosome-based cloning and expression vectors. Using clone-by-clone sequencing methodology, we obtained the complete genomic sequence of the centromeric region of rice (Oryza sativa) chromosome 8. Analysis of 1.97 Mb of contiguous nucleotide sequence revealed three large clusters of CentO satellite repeats (68.5 kb of 155-bp repeats) and >220 transposable element (TE)-related sequences; together, these account for approximately 60% of this centromeric region. The 155-bp repeats were tandemly arrayed head to tail within the clusters, which had different orientations and were interrupted by TE-related sequences. The individual 155-bp CentO satellite repeats showed frequent transitions and transversions at eight nucleotide positions. The 40 TE elements with highly conserved sequences were mostly gypsy-type retrotransposons. Furthermore, 48 genes, showing high BLAST homology to known proteins or to rice full-length cDNAs, were predicted within the region; some were close to the CentO clusters. We then performed a genome-wide survey of the sequences and organization of CentO and RIRE7 families. Our study provides the complete sequence of a centromeric region from either plants or animals and likely will provide insight into the evolutionary and functional analysis of plant centromeres.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004084653

Download citation: RISBibTeXText

PMID: 15037733

DOI: 10.1105/tpc.019273


Related references

Composition and Structure of the Centromeric Region of Rice Chromosome 8. The Plant Cell 16(4): 967-976, 2004

Asymmetric distribution of gene expression in the centromeric region of rice chromosome 5. Frontiers in Plant Science 2: 16, 2011

Structure of repeated sequences in the centromeric region of the human Y chromosome. Development 101 Suppl: 93, 1987

A segment of the apospory-specific genomic region is highly microsyntenic not only between the apomicts Pennisetum squamulatum and buffelgrass, but also with a rice chromosome 11 centromeric-proximal genomic region. Plant Physiology 140(3): 963-971, 2006

A Segment of the Apospory-Specific Genomic Region Is Highly Microsyntenic Not Only between the Apomicts Pennisetum squamulatum and Buffelgrass, but Also with a Rice Chromosome 11 Centromeric-Proximal Genomic Region. Plant Physiology 140(3): 963-971, 2006

Molecular fine structure of the centromeric region of chromosome 21 analysis of nondisjunction events yielding down syndrome. American Journal of Human Genetics 39(3 Suppl.): A222, 1986

Single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato. Euphytica 210(3): 327-339, 2016

The structure of the centromeric region and chromosome behavior in the anaphase after incubating murine cells with the fluorochrome Hoechst 33258. Tsitologiia 35(1): 30-35, 1993

The structure of the centromeric region and chromosome behaviour in the anaphase after mouse cell incubation with fluorochrome Hoechst 33258. Tsitologiya 35(1): 30-35, 1993

Absence of chromosome 11 specific centromeric DNA in a constitutional ring chromosome with dispersed centromeric DNA in a family with Wilms' tumor. Cancer Genetics and Cytogenetics 56(1): 112-113, 1991

Juxtacentromeric region of human chromosome 21: a boundary between centromeric heterochromatin and euchromatic chromosome arms. Gene (Amsterdam) 312: 41-50, 17 July, 2003

Construction of an 800-kb contig in the near-centromeric region of the rice blast resistance gene Pi-ta2 using a highly representative rice BAC library. Molecular & General Genetics 254(6): 611-620, 1997

Construction of an 800-kb contig in the near-centromeric region of the rice blast resistance genePi-ta2using a highly representative rice BAC library. Molecular Genetics and Genomics 254(6): 611-620, 1997

Analysis of the centromeric region of chromosome 21. Cytogenetics & Cell Genetics 77(Suppl. 1): 15, 1997

Sequence composition of a centromeric chromosome 19 probe. American Journal of Human Genetics 59(4 Suppl. ): A358, 1996