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Construction of pVAX1-SAG2 plasmid encoding the SAG2 protein of Toxoplasma gondii and the immune responses induced in mice



Construction of pVAX1-SAG2 plasmid encoding the SAG2 protein of Toxoplasma gondii and the immune responses induced in mice



Chinese Journal of Zoonoses 20(8): 658-661



The aims of this study were to construct the pVAX1-SAG2 recombinant plasmid encoding the SAG2 protein of T. gondii, and to evaluate the immune responses in mice vaccinated with this DNA vaccine. The SAG2 encoding DNA fragment was obtained from the plasmid pGEM/SAG2 by double enzyme digestion, and inserted into the polylinker of eukaryotic expression plasmid pVAX1 to construct recombinant pVAX1-SAG2, which was transferred into the competent cell E. coli JM109.

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