Construction of plant expression plasmid vector containning the antisense gene CYP86MF and its application in the transformation of Chinese cabbage-pak-choi

Y.X.aoLin; Cao JiaShu

Journal of Zhejiang University Agriculture and Life Sciences 30(2): 179-184

2004


Accession: 004087029

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
The male-sterility-related gene CYP86MF, which encoded a plant cytochrome P450 was isolated in a previous study, based on which an antisense gene fragment of 438 bp was amplified from the 901st to 1338th base pairs. The fragment was introduced to pBI121 generating pBI35S-AMF which contained CaMV35S promoter, and then pBI35S-AMF was transferred into Agrobacterium tumefaciens strain LBA4404 and EHA105 through the assistant plasmid pRK2013/HB101 by triparental cross. Results of polymerase chain reaction amplification and restriction digestion showed that the fragment of antisense gene CYP86MF was introduced into pBI121 plasmid, and the expression vector pBI35S-AMF was transferred into A. tumefaciens successfully. The antisense gene fragment was transferred into Chinese cabbage (Brassica campestris subsp. chinensis [B. chinensis] cv. Shanghaiqing), and more than 130 KanR plantlets were obtained.