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Construction of plant expression vector of the coat protein gene of zucchini yellow mosaic virus from China



Construction of plant expression vector of the coat protein gene of zucchini yellow mosaic virus from China



Journal of Fruit Science 21(6): 579-581



Vector pPZCP-87 containing the coat protein gene of zucchini yellow virus (ZYMV) from China was digested by Sal I and BamH I and ligased to plant expression vector pBIN438 digested by the same enzymes. The recombinant plasmid was transferred into Escherichia coli DH 5 alpha. Polymerase chain reaction and double digestion test showed that the coat protein was constructed into the plant expression vector.

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Accession: 004087033

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