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Construction of targeting vector and expression of green fluorescent protein in the silkworm, Antheraea pernyi



Construction of targeting vector and expression of green fluorescent protein in the silkworm, Antheraea pernyi



Dna and Cell Biology 22(7): 441-446



The transcription initiation site of Anrheraea pernyi was identified, and a gene targeting vector was constructed. The green fluorescent protein (GFP) gene was inserted into this vector under the control of a fibroin promoter. This recombinant vector was used to target the GFP gene to the fibroin region of the silkworm genome. The DNA was injected into the testes during the pupae, and was also transferred into the egg via the sperm during fertilization. The analysis showed that the GFP gene was integrated into the flbroin gene on the genome by homologous recombination, and was expressed in the silk gland.

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Accession: 004087070

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PMID: 12932302

DOI: 10.1089/104454903322247316


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