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Construction of template for detecting changes of microsomal triglyceride transfer protein mRNA in liver of dairy cows



Construction of template for detecting changes of microsomal triglyceride transfer protein mRNA in liver of dairy cows



Chinese Journal of Veterinary Science and Technology 35(9): 731-734



The objective of this study was to construct a recombinant DNA fragment which can be used as a competitive template for detecting changes of MTP mRNA in the liver of dairy cows. A 194 bp fragment was cut from a 394 bp MTP recombinant plasmid by restriction enzyme Apa I. Then, the larger linear fragment was ligated by T4 DNA ligase.

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Accession: 004087071

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