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Construction of tender shoots cDNA library and preliminary analysis of expressed sequence tags sequencing of tea plant



Construction of tender shoots cDNA library and preliminary analysis of expressed sequence tags sequencing of tea plant



Journal of Tea Science 24(1): 18-22



The construction of the first tea (cv. Longjing 43) cDNA library in China and the analysis of expressed sequence tags ratio were reported. The total RNA was isolated from tender tea shoots using TRIZOL single-stem method followed by mRNA separation and double-stranded (ds) cDNA amplification by LD-PCR. After size fractionation, the ds-cDNA was ligated to gamma -TripEX2 and recombinant bacteriophages were packaged.

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