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Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2



Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2



Acta Crystallographica. Section D, Biological Crystallography 59(Pt 7): 1302-1305



In yeast, the second and the third reaction of the fatty-acid beta -oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain. Here, the purification, crystallization and X-ray diffraction analysis of the hydratase 2 domain (CtMfe2p(dh<sub>a+b</sub> Delta )) from Candida tropicalis Mfe2p is reported.

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Accession: 004092298

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PMID: 12832794

DOI: 10.1107/s090744490300982x


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