DNA fingerprinting of Ralstonia paucula by infrequent-restriction-site PCR and randomly amplified polymorphic DNA analysis
Moissenet, D.; Vu-Thien, H.; Benzerara, Y.; Arlet, G.
Journal of Clinical Microbiology 41(12): 5747-5749
ISSN/ISBN: 0095-1137 PMID: 14662974 DOI: 10.1128/jcm.41.12.5747-5749.2003
Ralstonia paucula (formerly CDC group IV c-2) is an environmental organism that can cause serious human infections, occasionally clusters of nosocomial infections. In the present work, 26 strains of R. paucula (4 from the American Centers for Disease Control and Prevention collection, 10 from the Belgian Laboratorium voor Microbiologie collection, and 12 French clinical isolates) were analyzed with infrequent-restriction-site PCR and randomly amplified polymorphic DNA analysis. Both techniques accurately distinguished between collection strains. Two close patterns obtained for all the French isolates suggested a clonal strain.