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Detection of Aspergillus fumigatus hyphae by solid phase cytometry

Detection of Aspergillus fumigatus hyphae by solid phase cytometry

Journal of Microbiological Methods 55(3): 557-564

ISSN/ISBN: 0167-7012

PMID: 14607399

DOI: 10.1016/s0167-7012(03)00174-x

A rapid method based on solid phase cytometry (SPC) for the detection of Aspergillus fumigatus hyphae is described. With an enzymatic "viability" staining procedure, fungal hyphae can be detected non-specifically within the hour. By combining this procedure with an immunofluorescence labelling, a distinction between Aspergillus spp. and other clinically important fungi is possible, except for Penicillium spp. due to cross-reactivity. To differentiate both genera, microcolonies are generated by incubation at 45 degrees C prior to viability staining. The latter approach in conjunction with immunofluorescence labelling allows a quasi-specific detection of A. fumigatus hyphae and has shown its applicability to samples of bronchoalveolar lavage liquid (BAL).

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Accession: 004098603

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