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Detection of Borrelia burgdorferi by species-specific and broad-range PCR of synovial fluid and synovial tissue of Lyme arthritis patients before and after antibiotic treatment



Detection of Borrelia burgdorferi by species-specific and broad-range PCR of synovial fluid and synovial tissue of Lyme arthritis patients before and after antibiotic treatment



Clinical and Experimental Rheumatology 21(2): 271-272



Synovial fluid samples from 11 patients with Lyme arthritis and from 15 controls with rheumatoid arthritis (n=10) or calcium pyrophosphate dihydrate deposition disease (n=5) were analysed for bacterial DNA by broad-range polymerase chain reaction (PCR) sequencing of a 16s ribosomal DNA gene fragment and by a B. burgdorferi flagellin-specific PCR All Lyme arthritis patients tested positive for B. burgdorferi by ELISA and the results were confirmed by Western blot.

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Accession: 004098620

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PMID: 12747294


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