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Detection of Broad bean wilt virus 1 and Broad bean wilt virus 2 in Japan



Detection of Broad bean wilt virus 1 and Broad bean wilt virus 2 in Japan



Annual Report of the Kanto Tosan Plant Protection Society (51): 43-48



Of the 34 isolates of broad bean wilt virus collected from 18 prefectures in Japan during 1962-99, and 122 field samples collected from 9 prefectures in 1997-2000, a large number of samples reacted strongly with an antiserum against broad bean wilt virus 2 (BBWV-2) in DAS-ELISA. One of these isolates that reacted strongly with the antiserum against BBWV-2 and also reacted weakly with an antiserum against broad bean wilt virus 1 (BBWV-1), was used for further analysis.

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